PHF21A Antikörper (AA 1-97)

Produktnummer ABIN6719353

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PHF21A in WB, ELISA, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PHF21A Antikörper (AA 1-97) (ABIN6719353)

Target: PHF21A (PHD Finger Protein 21A (PHF21A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PHF21A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-PHF21A Antikörper

Bindungsspezifität: AA 1-97

Verwendungszweck: Anti-BHC80/PHF21A Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-BHC80/PHF21A Antibody (ABIN6719353). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human BHC80/PHF21A recombinant protein (Position: M1-Q97).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human,Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Nagase T, Kikuno R, Hattori A, Kondo Y, Okumura K, Ohara O (2000). "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (6): 347-55. 2. Iwase S, Januma A, Miyamoto K, Shono N, Honda A, Yanagisawa J, Baba T (Sep 2004). "Characterization of BHC80 in BRAF-HDAC complex, involved in neuron-specific gene repression". Biochem. Biophys. Res. Commun. 322 (2): 601-8.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PHF21A

Target: PHF21A (PHD Finger Protein 21A (PHF21A))

Andere Bezeichnung: PHF21A

Hintergrund:

Synonyms: PHD finger protein 21A, BHC80a, BRAF35-HDAC complex protein BHC80, PHF21A, BHC80, KIAA1696, BM-006

Tissue Specificity: Highly expressed in brain. Expressed at much lower level in other tissues.

Background: PHD finger protein 21A is a protein that in humans is encoded by the PHF21A gene. The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC, see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS).

Molekulargewicht: 75-95 kDa

Gen-ID: 51317

Pathways: Feeding Behaviour