OPTN Antikörper (AA 241-577)

Produktnummer ABIN6719352

Dieses Anti-OPTN-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von OPTN in WB und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für OPTN Antikörper (AA 241-577) (ABIN6719352)

Target: OPTN (Optineurin (OPTN))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser OPTN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: 3D8

Produktdetails anti-OPTN Antikörper

Bindungsspezifität: AA 241-577

Verwendungszweck: Anti-Optineurin/OPTN Antibody Picoband® (monoclonal, 3D8)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Optineurin/OPTN Antibody Picoband® (monoclonal, 3D8) (ABIN6719352). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Optineurin recombinant protein (Position: R241-I577). Human Optineurin shares 82% amino acid (aa) sequence identity with both mouse and rat Optineurin.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Johnson L, et al. Screening for OPTN mutations in a cohort of British amyotrophic lateral sclerosis patients. Neurobiol Aging, 2012 Dec. 2. Michou L, et al. Genetic association study of UCMA/GRP and OPTN genes (PDB6 locus) with Paget's disease of bone. Bone, 2012 Oct. 3. Weishaupt JH, et al. A novel optineurin truncating mutation and three glaucoma-associated missense variants in patients with familial amyotrophic lateral sclerosis in Germany.

Kommentare:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu OPTN

Target: OPTN (Optineurin (OPTN))

Andere Bezeichnung: OPTN

Hintergrund:

Synonyms: Optineurin, E3-14.7K-interacting protein, FIP-2, Huntingtin yeast partner L, Huntingtin-interacting protein 7,HIP-7, Huntingtin-interacting protein L, NEMO-related protein, Optic neuropathy-inducing protein, Transcription factor IIIA-interacting protein, TFIIIA-IntP, OPTN, FIP2, GLC1E, HIP7, HYPL, NRP

Tissue Specificity: Present in aqueous humor of the eye. Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta.

Background: OPTN is also known as NRP, FIP2 or HYPL. This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.

Molekulargewicht: 66 kDa

Gen-ID: 10133

Pathways: M Phase