CKM Antikörper (AA 3-381)

Produktnummer ABIN6719307

Dieses Anti-CKM-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CKM in WB, IHC und ELISA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für CKM Antikörper (AA 3-381) (ABIN6719307)

Target: CKM (Creatine Kinase, Muscle (CKM))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CKM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-CKM Antikörper

Bindungsspezifität: AA 3-381

Verwendungszweck: Anti-Creatine Kinase MM/CKM Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Creatine Kinase MM/CKM Antibody Picoband® (ABIN6719307). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Creatine Kinase MM recombinant protein (Position: F3-K381).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Bailly, J., MacKenzie, A. E., Leblond, S., Korneluk, R. G. Assessment of a creatine kinase isoform M defect as a cause of myotonic dystrophy and the characterization of two novel CKMM polymorphisms. Hum. Genet. 86: 457-462, 1991. 2. Dawson, D. M., Eppenberger, H. M., Eppenberger, M. E. Multiple molecular forms of creatine kinases. Ann. N.Y. Acad. Sci. 151: 616-626, 1968.

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CKM

Target: CKM (Creatine Kinase, Muscle (CKM))

Andere Bezeichnung: CKM

Hintergrund:

Synonyms: Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type , CPK-M, M-CK, CKM, CKMM

Background: Creatine kinase, muscle also known as CKM is a creatine kinase that in humans is encoded by the CKM gene. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Molekulargewicht: 43 kDa

UniProt: P06732