ACTN3 Antikörper (C-Term)

Produktnummer ABIN6719273

Der Maus-Monoklonal Anti-ACTN3-Antikörper wurde für WB und IHC validiert. Er ist geeignet zum Nachweis von ACTN3 in Proben aus Maus und Ratte. }

Kurzübersicht für ACTN3 Antikörper (C-Term) (ABIN6719273)

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Reaktivität: Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACTN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 9B5

Produktdetails anti-ACTN3 Antikörper

Bindungsspezifität: AA 574-617, C-Term

Verwendungszweck: Anti-ACTN3 Antibody (monoclonal, 9B5)

Sequenz: EADRERGAIM GIQGEIQKIC QTYGLRPCST NPYITLSPQD INT

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ACTN3 Antibody (monoclonal, 9B5) (ABIN6719273). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3, different from the related mouse sequence by five amino acids.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL

Kommentare:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ACTN3

Target: ACTN3 (Actinin, alpha 3 (ACTN3))

Andere Bezeichnung: ACTN3

Hintergrund:

Synonyms: Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, ACTN3

Tissue Specificity: Expressed only in a subset of type 2 skeletal muscle fibers.

Background: Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants, the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.

Molekulargewicht: 103 kDa

Gen-ID: 89

UniProt: Q08043

Pathways: Cell-Cell Junction Organization