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Vimentin Antikörper

VIM Reaktivität: Human WB, ELISA, FM Wirt: Huhn Polyclonal unconjugated
Produktnummer ABIN6699200
  • Target Alle Vimentin (VIM) Antikörper anzeigen
    Vimentin (VIM)
    Reaktivität
    • 333
    • 127
    • 126
    • 61
    • 59
    • 44
    • 43
    • 37
    • 30
    • 22
    • 20
    • 14
    • 13
    • 8
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 190
    • 130
    • 8
    • 6
    • 4
    • 4
    • 2
    Huhn
    Klonalität
    • 197
    • 146
    Polyklonal
    Konjugat
    • 200
    • 19
    • 13
    • 13
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Dieser Vimentin Antikörper ist unkonjugiert
    Applikation
    • 282
    • 132
    • 100
    • 99
    • 95
    • 65
    • 49
    • 40
    • 39
    • 28
    • 23
    • 20
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
    Kreuzreaktivität
    Rind (Kuh), Human, Maus, Ratte (Rattus)
    Kreuzreaktivität (Details)
    Cross reactivity with Vimentin from other species has not been determined.
    Aufreinigung
    Anti-Vimentin antibody is directed against human Vimentin proteins. The antibody was purified from chicken eggs as an IgY fraction. Reactivity is expected from human, mouse and rat sources.
    Immunogen

    Immunogen: Anti-Vimentin Monoclonal Antibody was produced in chicken by repeated immunizations with recombinant human vimentin extensively purified from E. coli.

    Immunogen Type: Recombinant Protein

    Isotyp
    IgG
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  • Applikationshinweise

    Application Note: Anti-Vimentin antibody is suitable for use in ELISA and Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to the vimentin proteins in Western blots in the appropriate cell lysate or extract.

    ELISA Dilution: 1:10,000

    Western Blot Dilution: 1:1000

    IF Microscopy Dilution: 1:500

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
  • Target
    Vimentin (VIM)
    Andere Bezeichnung
    Vimentin (VIM Produkte)
    Synonyme
    CTRCT30 antikoerper, cb28 antikoerper, vime antikoerper, vim antikoerper, vim1 antikoerper, vim2 antikoerper, VIM antikoerper, Vimentin antikoerper, vim4 antikoerper, vimentin antikoerper, vimentin L homeolog antikoerper, vimentin S homeolog antikoerper, VIM antikoerper, Vim antikoerper, vim antikoerper, vim.L antikoerper, vim.S antikoerper
    Hintergrund

    Synonyms: VIM

    Background: Vimentin antibody recognizes Vimentin which is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration. Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness. Vimentin antibody is ideal for investigators involved in Neuroscience, Cell Migration and Cancer Research.

    Gene Name: VIM

    Gen-ID
    7431
    UniProt
    P08670
    Pathways
    Caspase Kaskade in der Apoptose
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