BrdU Antikörper (Biotin)

Produktnummer ABIN669248

Der Kaninchen Polyklonal Anti-BrdU-Antikörper wurde für IHC (p), IHC (fro) und ELISA validiert. Er ist geeignet, BrdU in Proben von zu detektieren.

Kurzübersicht für BrdU Antikörper (Biotin) (ABIN669248)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BrdU Antikörper ist konjugiert mit Biotin

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA

Produktdetails anti-BrdU Antikörper (Biotin)

Kreuzreaktivität: Human, Ratte

Kreuzreaktivität (Details): BrdU

Aufreinigung: Purified by Protein A.

Immunogen: BrdU conjugated to KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC-P 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Andere Bezeichnung: BrdU

Substanzklasse: Chemical

Hintergrund:

Synonyms: Bromodeoxyuridine, Bromodeoxyuridine, 5-Bromo-2-deoxyuridine, 5-BrdU, proliferation Marker.

Background: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis