CRISPR-Cas9 (C-Term) Antikörper (DyLight 800)

Produktnummer ABIN6658290

Hersteller Produkt- Nr.: 600-445-gk0

This anti- antibody (ABIN6658290) is a Rabbit Polyclonal antibody detecting in WB, ELISA, FM. Suitable for .

Kurzübersicht für CRISPR-Cas9 (C-Term) Antikörper (DyLight 800) (ABIN6658290)

Target: CRISPR-Cas9

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: DyLight 800

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Produktdetails

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-445-gk0

Hersteller: Rockland

Verwendungszweck: Cas 9 Antibody DyLight™800 Conjugated

Kreuzreaktivität (Details): Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9.

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

Isotyp: IgG

Labeling Ratio: 2.4

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:25,000-1:30,000

IF_Microscopy_Dilution: 1 μg/mL

Western_Blot_Dilution: 1 μg/mL

Kommentare:

Suggested Applications: User Optimized
Cas9 DyLight™800 Conjugated Antibody has been tested for use in ELISA and western blotting and suitable in Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: CRISPR-Cas9

Andere Bezeichnung: cas9

Hintergrund:

Synonyms: rabbit anti-Cas9 antibody DyLight™ 800 conjugation, DyLight™ 800 conjugated rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody

Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.

Gene Name: cas9

Gen-ID: 901176

NCBI Accession: NP_269215

UniProt: Q99ZW2