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CAMK2A Antikörper

Dieses Anti-CAMK2A-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CAMK2A in WB, IHC, ELISA, IP, IF und FM. Geeignet für Ratte.
Rockland
Produktnummer ABIN6658262
Hersteller Produkt- Nr.: 200-301-f01

Kurzübersicht für CAMK2A Antikörper (ABIN6658262)

Target

Alle CAMK2A Antikörper anzeigen
CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reaktivität

  • 65
  • 53
  • 46
  • 3
  • 1
  • 1
  • 1
  • 1
Ratte

Wirt

  • 73
  • 20
Maus

Klonalität

  • 61
  • 32
Monoklonal

Konjugat

  • 65
  • 6
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser CAMK2A Antikörper ist unkonjugiert

Applikation

  • 73
  • 39
  • 31
  • 17
  • 15
  • 10
  • 9
  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Klon

22B1
  • Hersteller Produkt- Nr.

    200-301-f01

    Hersteller

    Rockland

    Verwendungszweck

    CaMKII Antibody

    Kreuzreaktivität (Details)

    This monoclonal antibody is specific for CaMKII protein.

    Aufreinigung

    Anti-CaMKII Antibody was purified by Protein G chromatography.

    Sterilität

    Sterile filtered

    Immunogen

    CaMKII Antibody was produced in mice by repeated immunizations with synthetic peptide of rat CaMKII.

    Isotyp

    IgG1
  • Applikationshinweise

    Immunoprecipitation_Dilution: User Optimized

    ELISA_Dilution: 1:200

    IF_Microscopy_Dilution: User Optimized

    Western_Blot_Dilution: 1 μg/mL

    Kommentare

    Anti-CaMKII Antibody is tested by IF, IP, WB, IHC, and ELISA. Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β). Specific conditions for reactivity should be optimized by the end user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

    Andere Bezeichnung

    Camk2a

    Hintergrund

    Synonyms: CamK2, CamK2A, CamK2B, CamK2D, CamK2G, CAMKA, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha

    Background: CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site. The binding of Ca2+ auto inhibitory effect and activates the kinase . This kinase activation results in autophosphorylation at threonine 286. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities.

    Gene Name: Camk2a

    Gen-ID

    25400

    NCBI Accession

    NP_037052

    UniProt

    P11275

    Pathways

    WNT Signalweg, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
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