CAMK2A Antikörper

Produktnummer ABIN6658151

Hersteller Produkt- Nr.: 200-301-f00

Dieses Anti-CAMK2A-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CAMK2A in WB, IHC, ELISA, IP, IF und FM. Geeignet für Ratte.

Kurzübersicht für CAMK2A Antikörper (ABIN6658151)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CAMK2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Klon: 6G9

Produktdetails anti-CAMK2A Antikörper

Hersteller Produkt- Nr.: 200-301-f00

Hersteller: Rockland

Verwendungszweck: CaMKII Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with CaMKII from mouse, rat, and bovine based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-CaMKII Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: CaMKII Antibody was produced in mice by repeated immunizations with a partially purified rat CaMKII.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: 1:2000

IF_Microscopy_Dilution: 1:50

Western_Blot_Dilution: 0.1 μg/mL (1:10,000)

Kommentare:

Anti-CaMKII Antibody has been tested in IF, IP, WB, IHC and RIA/ELISA. Antibody is provided in PBS pH 7.4. Expect a band approximately ~50-60kDa protein, corresponding to the molecular mass of CaMKII α-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: See application note.

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Andere Bezeichnung: Camk2a

Hintergrund:

Synonyms: CamK2, CamK2A, CamK2B, CamK2D, CamK2G, CAMKA, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha

Background: CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site. The binding of Ca2+ auto inhibitory effect and activates the kinase . This kinase activation results in autophosphorylation at threonine 286. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities.

Gene Name: Camk2a

Gen-ID: 25400

NCBI Accession: NP_037052

UniProt: P11275

Pathways: WNT Signalweg, Interferon-gamma Pathway, Myometrial Relaxation and Contraction