GADD45G Antikörper (AA 142-159)

Produktnummer ABIN6658028

Hersteller Produkt- Nr.: 100-401-863

Dieses Anti-GADD45G-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GADD45G in WB, ELISA und IP. Geeignet für Maus.

Kurzübersicht für GADD45G Antikörper (AA 142-159) (ABIN6658028)

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GADD45G Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-GADD45G Antikörper

Bindungsspezifität: AA 142-159

Hersteller Produkt- Nr.: 100-401-863

Hersteller: Rockland

Verwendungszweck: GADD45 gamma Antibody

Kreuzreaktivität (Details): Reactivity occurs against mouse PI3K p110d subunit.

Aufreinigung: This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.

Sterilität: Sterile filtered

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 142-159 of Mouse GADD45 gamma. Swiss Prot accession number Q9Z111. This sequence represents the C-terminal end of the protein.  Mouse and rat GADD45 g show 100% sequence homology.   A single amino acid change is found in the human sequence (see below).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: Use 10 μL

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 1:100 - 1:500

Other: User Optimized

Kommentare:

This antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Reactivity in other immunoassays is unknown. This product was assayed by western blotting after immunoprecipitation of mouse GADD45 gamma from a lysate containing over expressed protein. A 1:100 to 1:500 dilution shows reactivity with a band at 19.2kDa corresponding to mouse GADD45 gamma. Use 10 μL for immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu GADD45G

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Andere Bezeichnung: GADD45 gamma

Hintergrund:

Synonyms: rabbit anti-GADD45 gamma Antibody, Cytokine responsive protein CR6 antibody, DDIT2 antibody, GADD45G antibody antibody, Growth arrest and DNA damage inducible gamma antibody, GRP17 antibody

Background: GADD45 gamma (Growth arrest and DNA-damage-inducible protein also known as cytokine responsive protein CR6) is derived from a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45 gamma is highly expressed in placenta.

Gene Name: GADD45 gamma

Gen-ID: 23882

NCBI Accession: NP_035947

UniProt: Q9Z111

Pathways: Zellzyklus