LAMP2 Antikörper

Produktnummer ABIN6657933

Hersteller Produkt- Nr.: 200-501-g10

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch LAMP2 in WB, IP, IF und FM. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für LAMP2 Antikörper (ABIN6657933)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser LAMP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Klon: GL2A7

Produktdetails anti-LAMP2 Antikörper

Hersteller Produkt- Nr.: 200-501-g10

Hersteller: Rockland

Verwendungszweck: Lamp2 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with LAMP2 from mouse and rabbit based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-LAMP2 Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: LAMP2 Antibody was produced in rat by repeated immunizations with purified preparation of mouse liver lysosomal membranes.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

IF_Microscopy_Dilution: 1:500- 1:1000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-LAMP2 Antibody is tested for use in WB, IF microscopy, and IP. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Andere Bezeichnung: LAMP2

Hintergrund:

Synonyms: CD107b, Igp110, Igp2, Lamp2C, LampB, MAC3, Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, Lysosomal membrane glycoprotein type B, LGP-B

Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology. They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane. Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine based motif (YXXI). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome. This stimulation has also been shown to have an associated release of histamine, leukotriene C and prostaglandin D, which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells. They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation. Anti-LAMP2 antibody is ideal for investigators involved in cytokines, growth factors and cancer research, as well as, Chaperones and Organelle Markers research.

Gene Name: LAMP2

Gen-ID: 16784

NCBI Accession: NP_001017959

UniProt: P17047

Pathways: Autophagie