SIX3 Antikörper (Internal Region)

Produktnummer ABIN6657913

Hersteller Produkt- Nr.: 600-401-a26s

Der Kaninchen Polyklonal Anti-SIX3-Antikörper wurde für WB, ELISA, IHC und FM validiert. Er ist geeignet, SIX3 in Proben von Maus zu detektieren. Es sind 6+ Publikationen verfügbar.

Kurzübersicht für SIX3 Antikörper (Internal Region) (ABIN6657913)

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIX3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Produktdetails anti-SIX3 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-a26s

Hersteller: Rockland

Verwendungszweck: Six3 Antibody

Kreuzreaktivität (Details): This antibody reacts with mouse Six3.

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000-1:15,000

Immunohistochemistry_Dilution: 1:100-1:250

IF_Microscopy_Dilution: 1:200 - 1:1,000

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Kommentare:

Suggested Applications: IF, Multiplex
This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 3 months

Referenzen für anti-SIX3 Antikörper (ABIN6657913)

Brignani, Raj, Schmidt, Düdükcü, Adolfs, De Ruiter, Rybiczka-Tesulov, Verhagen, van der Meer, Broekhoven, Moreno-Bravo, Grossouw, Dumontier, Cloutier, Chédotal, Pasterkamp: "Remotely Produced and Axon-Derived Netrin-1 Instructs GABAergic Neuron Migration and Dopaminergic Substantia Nigra Development." in: Neuron, Vol. 107, Issue 4, pp. 684-702.e9, (2020) (PubMed).

Roy, Murphy, Deng, MacDonald, Bammler, Aldinger, Glass, Millen: "PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice." in: eLife, Vol. 8, (2020) (PubMed).

Li, Gao, Wang, Song, Xu, Xie, Zhou, Pan, Peng, Zhang, Ge, Zhong: "Generation and Characterization of Induced Pluripotent Stem Cells and Retinal Organoids From a Leber's Congenital Amaurosis Patient With Novel RPE65 Mutations." in: Frontiers in molecular neuroscience, Vol. 12, pp. 212, (2019) (PubMed).

Xu, Liang, Song, Zhang, Lindtner, Li, Wen, Liu, Guo, Qi, Wang, Wang, Li, You, Wang, Chen, Feng, Rubenstein, Yang: "SP8 and SP9 coordinately promote D2-type medium spiny neuron production by activating Six3 expression." in: Development (Cambridge, England), Vol. 145, Issue 14, (2018) (PubMed).

Li, Xie, He, Zhou, Gao, Liu, Pan, Ge, Peng, Zhong: "Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells." in: Stem cells international, Vol. 2018, pp. 4968658, (2018) (PubMed).

Diacou, Zhao, Zheng, Cvekl, Liu: "Six3 and Six6 Are Jointly Required for the Maintenance of Multipotent Retinal Progenitors through Both Positive and Negative Regulation." in: Cell reports, Vol. 25, Issue 9, pp. 2510-2523.e4, (2018) (PubMed).

Details zu SIX3

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Andere Bezeichnung: Six3

Hintergrund:

Synonyms: rabbit anti-Six3 antibody, Six-3, Six 3, Homeobox protein SIX3, Sine oculis homeobox homolog 3

Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Gene Name: Six3

Gen-ID: 20473, 59939908

UniProt: Q62233

Pathways: Protein targeting to Nucleus