SIX3 Antikörper (Internal Region)

Produktnummer ABIN6657911

Produktdetails anti-SIX3 Antikörper

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Bindungsspezifität: Internal Region

Reaktivität: Maus

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser SIX3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Aufreinigung: This product was purified by Protein A chromatography from monospecific antiserum.  This antibody reacts with mouse Six3.   Expect partial reactivity with Six3 from chicken and Xenopus sources based on 95% and 85% homologies, respectively.  Cross-reactivity with Six3 from other sources has not been determined.

Immunogen:

Immunogen: This Protein A purified antibody was prepared from whole guinea pig serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200

Application Note: This Protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

ELISA Dilution: 1:5,000 - 1:25,000

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:200 - 1:1,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Referenzen für anti-SIX3 Antikörper (ABIN6657911)

Lipiec, Bem, Koziński, Chakraborty, Urban-Ciećko, Zajkowski, Dąbrowski, Szewczyk, Toval, Ferran, Nagalski, Wiśniewska: "TCF7L2 regulates postmitotic differentiation programmes and excitability patterns in the thalamus." in: Development (Cambridge, England), Vol. 147, Issue 16, (2021) (PubMed).

OBrien, Guo, Bahrami-Samani, Park, Hasso, Lee, Fang, Kim, Guo, Hong, Peterson, Lozanoff, Raviram, Ren, Fogelgren, Smith, Valouev, McMahon: "Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies." in: PLoS genetics, Vol. 14, Issue 1, pp. e1007181, (2018) (PubMed).

Details zu SIX3

Target: SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

Andere Bezeichnung: SIX3 (SIX3 Produkte)

Synonyme: HPE2 antikoerper, SIX3 antikoerper, cb347 antikoerper, six3.2 antikoerper, six6 antikoerper, E130112M24Rik antikoerper, Six3a antikoerper, Six3alpha antikoerper, Six3b antikoerper, Six3beta antikoerper, six3 antikoerper, six3.1 antikoerper, wu:fc10a08 antikoerper, SIX homeobox 3 antikoerper, SIX homeobox 3b antikoerper, sine oculis-related homeobox 3 antikoerper, SIX homeobox 3a antikoerper, sine oculis homeobox homolog 3 antikoerper, SIX3 antikoerper, six3b antikoerper, Six3 antikoerper, six3a antikoerper, six3 antikoerper

Hintergrund:

Synonyms: guinea pig anti-SIX 3 antibody, Six3, Sine oculis homeobox homolog 3

Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

Gene Name: SIX3

Gen-ID: 20473, 59939908

UniProt: Q62233

Pathways: Protein targeting to Nucleus