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TCRb Antikörper (FITC)

Dieses Hamster Monoklonal-Antikörper erkennt spezifisch TCRb in FACS, IP und FM. Er zeigt eine Reaktivität gegenüber Maus.
Rockland
Produktnummer ABIN6657906
Hersteller Produkt- Nr.: 200-b02-n92

Kurzübersicht für TCRb Antikörper (FITC) (ABIN6657906)

Target

Alle TCRb Antikörper anzeigen
TCRb (T Cell Receptor beta Chain (TCRb))

Reaktivität

  • 13
  • 9
Maus

Wirt

  • 13
  • 9
Hamster

Klonalität

  • 13
  • 9
Monoklonal

Konjugat

  • 8
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
Dieser TCRb Antikörper ist konjugiert mit FITC

Applikation

  • 10
  • 9
  • 6
  • 6
  • 2
  • 1
  • 1
Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Klon

H57-597
  • Hersteller Produkt- Nr.

    200-b02-n92

    Hersteller

    Rockland

    Verwendungszweck

    TCRbeta Fluorescein Antibody

    Kreuzreaktivität (Details)

    Cross reactivity with TCRbeta from other sources has not been tested.

    Aufreinigung

    Fluorescein conjugated TCRbeta Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse TCRbeta.

    Sterilität

    Sterile filtered

    Immunogen

    Anti-TCRbeta Antibody (Monoclonal) was produced by repeated immunizations with TCRbeta antigen.

    Isotyp

    IgG

    Labeling Ratio

    2-8
  • Applikationshinweise

    Immunoprecipitation_Dilution: User Optimized

    Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

    IF_Microscopy_Dilution: User Optimized

    Other: Sufficient to run approximately 500 tests

    Kommentare

    Anti-TCR beta is tested for FLOW and useful for Immunofluorescence and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.1 % Gelatin

    Preservative: 0.09 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

    Haltbarkeit

    6 months
  • Target

    TCRb (T Cell Receptor beta Chain (TCRb))

    Andere Bezeichnung

    Tcrb

    Hintergrund

    Synonyms: Tib, TCRbeta, T cell receptor beta chain, Tcrb

    Background: The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma and delta protein chains, known as the gamma-delta TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the gamma-delta TCR.

    Gene Name: Tcrb

    Gen-ID

    21577
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