CD86 Antikörper (APC)

Produktnummer ABIN6657892

Hersteller Produkt- Nr.: 200-526-n90

Der Ratte Monoklonal Anti-CD86-Antikörper wurde für FACS, IHC und FM validiert. Er ist geeignet, CD86 in Proben von Maus zu detektieren.

Kurzübersicht für CD86 Antikörper (APC) (ABIN6657892)

Target: CD86

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD86 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Klon: GL-1

Produktdetails anti-CD86 Antikörper (APC)

Hersteller Produkt- Nr.: 200-526-n90

Hersteller: Rockland

Verwendungszweck: CD86 Allophycocyanin Antibody

Kreuzreaktivität (Details): Cross reactivity with CD86 from other sources has not been tested.

Aufreinigung: Allophycocyanin conjugated CD86 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD86.

Sterilität: Sterile filtered

Immunogen: Anti-CD86 Antibody (Monoclonal) was produced by repeated immunizations with CD86 antigen.

Isotyp: IgG2a kappa

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Kommentare:

Anti-CD86 is tested for FLOW and useful for Immunohistochemistry and Immunofluorescence using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Haltbarkeit: 6 months

Details zu CD86

Target: CD86

Andere Bezeichnung: CD86

Hintergrund:

Synonyms: T-lymphocyte activation antigen CD86, Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1, CD86, Cd86

Background: Anti-CD86 reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. The GL-1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.

Gene Name: Cd86

Gen-ID: 12524

NCBI Accession: NP_062261

UniProt: P42082

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation