NK-1.1/CD161c Antikörper (APC)

Produktnummer ABIN6657869

Hersteller Produkt- Nr.: 200-326-n93

Der APC-konjugierte Maus Monoklonal anti-NK-1.1/CD161c Antikörper (Klon PK136) (ABIN6657869) detektiert spezifisch NK-1.1/CD161c in FACS, IP, IHC und FM. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für NK-1.1/CD161c Antikörper (APC) (ABIN6657869)

Target: NK-1.1/CD161c (NK-1.1)

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NK-1.1/CD161c Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Klon: PK136

Produktdetails anti-NK-1.1/CD161c Antikörper (APC)

Hersteller Produkt- Nr.: 200-326-n93

Hersteller: Rockland

Verwendungszweck: NK1.1 Allophycocyanin Antibody

Aufreinigung: Allophycocyanin conjugated NK1.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse NK1.

Sterilität: Sterile filtered

Immunogen: Anti-NK1.1 Antibody (Monoclonal) was produced by repeated immunizations with NK1.1 antigen.

Isotyp: IgG2a kappa

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Kommentare:

Anti-NK1.1 is tested in Flow Cytometry useful for Immunohistochemistry, Immunoprecipitation, Immunofluorescence using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu NK-1.1/CD161c

Target: NK-1.1/CD161c (NK-1.1)

Andere Bezeichnung: NK1.1

Hintergrund:

Synonyms: Killer cell lectin-like receptor subfamily B member 1C, CD161 antigen-like family member C, Lymphocyte antigen 55c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C, Natural killer cell surface protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c

Background: The PK136 antibody is specific for mouse NK1.1, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. NK1.1 is prominently expressed on natural killer (NK) cells, and is correlated with NK cytotoxic effects toward virus-infected cells and tumor cells. NK1.1 is expressed on subsets of NKT cells in certain mouse strains (C57BL/6, FVB/N, and NZB), yet absent from others (AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129). Putative subsets of NK cells and their expression of NK1.1 antigen are of continuing interest, including NK1.1+/CD117+ (c-Kit) cells reported to be immunosuppressive for CD8+ T cells in a mechanism involving PD-1 and PD-L1. The PK136 antibody may be used for detection of NK1.1 expression on mouse strains including CE, B6, NZB, C58, Ma/My, ST, SJL, and FVB. The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of NK1.1.

Gene Name: Klrb1c

Gen-ID: 17059

NCBI Accession: NP_001153376

UniProt: P27814