CD80 Antikörper (PE)

Produktnummer ABIN6657769

Hersteller Produkt- Nr.: 200-b08-n89

Der Hamster Monoklonal Anti-CD80-Antikörper wurde für FACS, IHC, IP und FM validiert. Er ist geeignet, CD80 in Proben von Maus zu detektieren.

Kurzübersicht für CD80 Antikörper (PE) (ABIN6657769)

Target: CD80

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD80 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Klon: 16-10A1

Produktdetails anti-CD80 Antikörper (PE)

Hersteller Produkt- Nr.: 200-b08-n89

Hersteller: Rockland

Verwendungszweck: CD80 Phycoerythrin Antibody

Kreuzreaktivität (Details): Cross reactivity with CD80 from other sources has not been tested.

Aufreinigung: Phycoerythrin conjugated CD80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD80.

Sterilität: Sterile filtered

Immunogen: Anti-CD80 Antibody (Monoclonal) was produced by repeated immunizations with CD80 antigen.

Isotyp: IgG

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Kommentare:

Anti-CD80 is tested for FLOW and useful for Immunohistochemistry, Immunofluorescence, and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu CD80

Target: CD80

Andere Bezeichnung: CD80

Hintergrund:

Synonyms: T-lymphocyte activation antigen CD80, Activation B7-1 antigen, B7, CD80, Cd80

Background: Anti-CD80 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

Gene Name: Cd80

Gen-ID: 12519

NCBI Accession: NP_033985

UniProt: Q00609

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints