CD80 Antikörper (APC)

Produktnummer ABIN6657767

Hersteller Produkt- Nr.: 200-b26-n89

Dieses Anti-CD80-Antikörper ist ein Hamster Monoklonal-Antikörper zur Detektion von CD80 in FACS, IHC und IP. Geeignet für Maus.

Kurzübersicht für CD80 Antikörper (APC) (ABIN6657767)

Target: CD80

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD80 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Klon: 16-10A1

Produktdetails anti-CD80 Antikörper (APC)

Hersteller Produkt- Nr.: 200-b26-n89

Hersteller: Rockland

Verwendungszweck: CD80 Allophycocyanin Antibody

Kreuzreaktivität (Details): CD80 has cross reactivity with canine, cross reactivity from other sources has not been tested.

Aufreinigung: Allophycocyanin conjugated CD80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD80.

Sterilität: Sterile filtered

Immunogen: Anti-CD80 Antibody (Monoclonal) was produced by repeated immunizations with CHO cell line transfected with mouse B7 (CD80).

Isotyp: IgG

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Kommentare:

Anti-CD80 is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu CD80

Target: CD80

Andere Bezeichnung: CD80

Hintergrund:

Synonyms: T-lymphocyte activation antigen CD80, Activation B7-1 antigen, B7, CD80, Cd80

Background: CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Gene Name: Cd80

Gen-ID: 12519

NCBI Accession: NP_033985

UniProt: Q00609

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints