RPS6 Antikörper (pSer244)

Produktnummer ABIN6657597

Hersteller Produkt- Nr.: 600-401-x26

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RPS6 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für RPS6 Antikörper (pSer244) (ABIN6657597)

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPS6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-RPS6 Antikörper

Bindungsspezifität: pSer244

Hersteller Produkt- Nr.: 600-401-x26

Hersteller: Rockland

Verwendungszweck: Ribosomal protein S6 Phospho S244 Antibody

Kreuzreaktivität (Details): Anti-Phospho-Ser244 Ribosomal Protein S6 Antibody is directed against Ribosomal Protein S6 phosphorylated at S244.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Anti-Phospho-Ser244 Ribosomal Protein S6 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Ser244 of mouse rpS6 conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Ribosomal Protein S6 pS244 Antibody is tested for Western Blots and is specific for the ~28 kDa rpS6 protein phosphorylated at Ser244. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Haltbarkeit: 12 months

Details zu RPS6

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Andere Bezeichnung: Ribosomal protein S6

Hintergrund:

Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33, rpS6

Background: Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5'-m7GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized . In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity. Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-γ), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)-Akt-mTOR pathway. Ribosomal Protein S6 pS244 Antibody is ideal for researchers interested in neuroscience research.

Gene Name: RPS6

Gen-ID: 20104

NCBI Accession: NP_033122

UniProt: P62754

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly