MYOD1 Antikörper (Internal Region)

Produktnummer ABIN6657593

Hersteller Produkt- Nr.: 200-301-h94

Produktdetails anti-MYOD1 Antikörper

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Bindungsspezifität: Internal Region

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MYOD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Hersteller Produkt- Nr.: 200-301-h94

Hersteller: Rockland

Verwendungszweck: MyoD Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-MyoD from Human, Cat, Rat, and Mouse based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-MyoD Antibody was purified by Protein G chromatography.

Immunogen: MyoD Antibody was produced in mice prepared by repeated immunizations with recombinant mouse MyoD protein at an internal amino acid sequence.

Klon: 5-8A

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: 1 μg/mL

Immunohistochemistry_Dilution: 5 μg/mL

Western_Blot_Dilution: 1 μg/mL

Kommentare:

Anti-MyoD Antibody is tested for use in WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, and IP. Expect a band approximately 45kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.02 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: MyoD (MYOD1 Produkte)

Hintergrund:

Synonyms: Myod, Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3

Background: Anti-MyoD Antibody detects MyoD. The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are: myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin). Anti-MyoD Antibody is ideal for investigators involved in cell cycle protein, cytokines and growth factors.

Gene Name: Myod1

Gen-ID: 17927

NCBI Accession: NP_034996

UniProt: P10085

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development