Lamin B1 Antikörper (C-Term)

Produktnummer ABIN6657537

Hersteller Produkt- Nr.: 600-401-p62

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Lamin B1 in WB und IHC. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für Lamin B1 Antikörper (C-Term) (ABIN6657537)

Target: Lamin B1 (LMNB1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Lamin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Lamin B1 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-401-p62

Hersteller: Rockland

Verwendungszweck: Lamin B1 Antibody

Kreuzreaktivität (Details): Anti-Lamin B1 antibody is directed against human Lamin B1 protein.

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Lamin B1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human Lamin B1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 0.5-1 μg/mL

Western_Blot_Dilution: 0.1-0.5 μg/mL

Other: Immunocytochemistry: 0.5-1 μg/mL

Kommentare:

Anti-Lamin B1 is tested for Immunohistochemistry-P, -F, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~66.4 kDa corresponding to the appropriate cell lysate or extract. WB tested in Human, Mouse, Rat, IHC-P (heat) and IHC-F in human and rat, ICC in human, and ICC/IF in human.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-Lamin B1 Antikörper (ABIN6657537)

Mastej, Axen, Wary, Minshall, Wary: "A requirement for Krüppel Like Factor-4 in the maintenance of endothelial cell quiescence." in: Frontiers in cell and developmental biology, Vol. 10, pp. 1003028, (2022) (PubMed).

Details zu Lamin B1

Target: Lamin B1 (LMNB1)

Andere Bezeichnung: Lamin B1

Hintergrund:

Synonyms: LMNB1, LMN2, LMNB, Lamin B1 antibody

Background: Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This antibody is suitable for researchers interested in chromatin antibody research.

Gene Name: LMNB1

Gen-ID: 4001

NCBI Accession: NP_001172028.11

UniProt: P20700

Pathways: Apoptose, Caspase Kaskade in der Apoptose