NLRP3 Antikörper (N-Term)

Produktnummer ABIN6657498

Hersteller Produkt- Nr.: 600-401-r14

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NLRP3 in WB, IHC, IF, FACS und FM. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für NLRP3 Antikörper (N-Term) (ABIN6657498)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NLRP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Produktdetails anti-NLRP3 Antikörper

Bindungsspezifität: N-Term

Hersteller Produkt- Nr.: 600-401-r14

Hersteller: Rockland

Verwendungszweck: NLRP3 Antibody

Kreuzreaktivität (Details): Anti-NLRP3 antibody is directed against human NLRP3 protein.

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: NLRP3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the N-terminal of human NLRP3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 1:100-1:500

Flow_Cytometry_Dilution: 1-3 μg/1x106 cells

IF_Microscopy_Dilution: 2 μg/mL

Western_Blot_Dilution: 0.5 μg/mL

Other: ICC: 2 μg/mL

Kommentare:

Anti-NLRP3 is tested for Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry -P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~118.2 kDa corresponding to the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 200μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-NLRP3 Antikörper (ABIN6657498)

Long, Kou, Li, Wu, Wang: "MiR-223-3p inhibits rTp17-induced inflammasome activation and pyroptosis by targeting NLRP3." in: Journal of cellular and molecular medicine, Vol. 24, Issue 24, pp. 14405-14414, (2021) (PubMed).

Details zu NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Andere Bezeichnung: NLRP3

Hintergrund:

Synonyms: Angiotensin/vasopressin receptor AII/AVP-like,Caterpiller protein 1.1,

Background: NLRP3(NLR FAMILY, PYRIN DOMAIN-CONTAINING 3),also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 (NOD-like receptor family, pryin domain containing 3) gene. The NLRP3 gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. And the NLRP3 gene is mapped on 1q44. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD (ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, Gross et al.showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance. This antibody is suitable for researchers interested in cancer research.

Gene Name: NLRP3

Gen-ID: 114548

NCBI Accession: NP_001073289

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome