MAP1LC3A Antikörper (N-Term)

Produktnummer ABIN6656617

Hersteller Produkt- Nr.: 200-401-h57

Der Kaninchen Polyklonal Anti-MAP1LC3A-Antikörper wurde für WB und IHC validiert. Er ist geeignet, MAP1LC3A in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für MAP1LC3A Antikörper (N-Term) (ABIN6656617)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP1LC3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-MAP1LC3A Antikörper

Bindungsspezifität: N-Term

Hersteller Produkt- Nr.: 200-401-h57

Hersteller: Rockland

Verwendungszweck: ATG8 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-ATG8 from Opossum and rat based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-ATG8 Antibody was purified by Protein G chromatography.

Immunogen: ATG8 Antibody was produced from whole rabbit serum prepared by repeated immunizations with at the n-terminus portion of human ATG8.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 5-10 μg/mL

Western_Blot_Dilution: 0.25-1 μg/mL

Kommentare:

Suggested Applications: IF
Anti-ATG8 antibody is tested by IHC-P and WB. Expect a band approximately 16 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-MAP1LC3A Antikörper (ABIN6656617)

Esposito, Falace, Wagner, Gal, Mei, Conti, Pisano, Aprile, Cerullo, De Fusco, Giovedì, Seibt, Magen, Polster, Eran, Stenton, Fiorillo, Ravid, Mayatepek, Hafner, Wortmann, Levanon, Marini, Mandel, Benfenati, Distelmaier, Fassio, Guerrini: "Biallelic DMXL2 mutations impair autophagy and cause Ohtahara syndrome with progressive course." in: Brain : a journal of neurology, Vol. 142, Issue 12, pp. 3876-3891, (2020) (PubMed).

Details zu MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Andere Bezeichnung: MAP1LC3A

Hintergrund:

Synonyms: Activating transcription factor 8

Background: Anti-ATG8 antibody detects human ATG8. MAP1A and MAP1B are microtubule-associated proteins made up of a heavy chain and multiple light chain components. One such light chain component associated with these proteins is ATG8. ATG8 (also called MAP1LC3A and APG8), an ~16KDa mammalian homolog of yeast Atg8, is a cytoplasmic protein that plays an essential role in the process and regulation of autophagy. ATG8 is an evolutionarily conserved protein that belongs to LC3 family. ATG8 interacts with various proteins in the cytoplasm including mammalian homologues of Atg4, Atg7, Atg3 and Atg12 and gets modified into LC3-I and LC3-II respectively. Both of these forms are suggested to be involved in the formation and intracellular trafficking of autophagosomes in mammalian cells. Anti-ATG8 antibody are ideal for investigators invovled in apoptosis research.

Gene Name: MAP1LC3A

Gen-ID: 84557

NCBI Accession: NP_115903

UniProt: Q9H492

Pathways: Autophagie