Choline Acetyltransferase Antikörper

Produktnummer ABIN6656077

Hersteller Produkt- Nr.: 600-101-d16

Der Ziege Polyklonal Anti-Choline Acetyltransferase-Antikörper wurde für WB und IHC validiert. Er ist geeignet, Choline Acetyltransferase in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Choline Acetyltransferase Antikörper (ABIN6656077)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Choline Acetyltransferase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Choline Acetyltransferase Antikörper

Hersteller Produkt- Nr.: 600-101-d16

Hersteller: Rockland

Verwendungszweck: ChAT (Choline Acetyltransferase) Antibody

Kreuzreaktivität (Details): Anti-ChAT antibody is directed against human ChAT protein.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:100

Kommentare:

Anti-ChAT antibody is tested by western blot and immunohistochemistry and suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western Blot in the appropriate lysate or tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 5 mg BSA

Preservative: 0.02 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-Choline Acetyltransferase Antikörper (ABIN6656077)

Liu, Kim, Kim, Zhang, Bao, Denaxa, Lim, Kim, Liu, Wickersham, Pachnis, Hattar, Song, Brown, Blackshaw: "Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep." in: Nature, Vol. 548, Issue 7669, pp. 582-587, (2017) (PubMed).

Details zu Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Andere Bezeichnung: ChAT (Choline Acetyltransferase)

Hintergrund:

Synonyms: acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2

Background: ChAT antibody recognizes choline acetyltransferase which is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. ChAT is found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome. Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects. It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function. Anti-ChAT antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CHAT

Gen-ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development