SOD2 Antikörper

Produktnummer ABIN6656073

Hersteller Produkt- Nr.: 600-401-g14

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SOD2 in WB, IHC, ELISA und IP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SOD2 Antikörper (ABIN6656073)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SOD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Produktdetails anti-SOD2 Antikörper

Hersteller Produkt- Nr.: 600-401-g14

Hersteller: Rockland

Verwendungszweck: SOD2 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with SOD2 from Human, Rat, Mouse, Bovine, Canine, Chicken, Gerbil, Guinea Pig, Pig, Hamster, Monkey, Rabbit, Sheep, and Xenopus based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-SOD2 Antibody was purified by affinity chromatography.

Sterilität: Sterile filtered

Immunogen: SOD2 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against human SOD2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 0.2 μg/mL

Kommentare:

Anti-SOD2 Antibody is tested for use in WB, IP, and IHC. Expect a band approximately ~25kDa protein corresponding to the molecular mass of SOD2 in WB. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Andere Bezeichnung: Mn SOD

Hintergrund:

Synonyms: Manganese SOD, IPO B, Mn SOD, SOD2, Superoxide dismutase [Mn], mitochondrial

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. In general, SODs play a major role in antioxidant defense mechanisms. There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space. SOD3 can also be distinguished by its heparin-binding capacity.

Gene Name: SOD2

Gen-ID: 6648

NCBI Accession: NP_000627

UniProt: P04179

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling