GRP78 Antikörper

Produktnummer ABIN6656071

Hersteller Produkt- Nr.: 200-301-f37

Der Maus Monoklonal Anti-GRP78-Antikörper wurde für WB, ELISA, IF und FM validiert. Er ist geeignet, GRP78 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für GRP78 Antikörper (ABIN6656071)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GRP78 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Klon: 6H4-2G7

Produktdetails anti-GRP78 Antikörper

Hersteller Produkt- Nr.: 200-301-f37

Hersteller: Rockland

Verwendungszweck: GRP78 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with GRP78 from Human, Mouse, Rat, Cow, Cryphonectria parasitica, Hamster, Monkey, Rabbit, and Xenopus based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-GRP78 Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Grp78 Antibody was produced in mice by repeated immunizations with a his-tagged human GRP78.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:200

IF_Microscopy_Dilution: 1:100

Kommentare:

Anti-GRP78 Antibody has been tested in WB and IF/ICC. Expect a band approximately ~78kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-GRP78 Antikörper (ABIN6656071)

Hong, Tseng, Wala, Kim, Kynnap, Doshi, Kugener, Sandoval, Howard, Li, Yang, Tillgren, Ghandi, Sayeed, Deasy, Ward, McSteen, Labella, Keskula, Tracy, Connor, Clinton, Church, Crompton, Janeway et al.: "Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition. ..." in: eLife, Vol. 8, (2019) (PubMed).

Details zu GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Andere Bezeichnung: GRP78

Hintergrund:

Synonyms: BiP, Grp78, HspA5, MIF2, immunoglobulin heavy chain binding protein, 78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5

Background: GRP78 is a ubiquitously expressed, 78- kDa glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed. GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis. Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival. In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress, and its protein levels are reduced in the brains of Alzheimer's patients. Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs.

Gene Name: Hspa5

Gen-ID: 3309

NCBI Accession: NP_005338

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling