Vimentin Antikörper

Produktnummer ABIN6656056

Hersteller Produkt- Nr.: 200-301-e54

Der Maus Monoklonal anti-Vimentin Antikörper wird verwendet zum Nachweis von Vimentin in Proben von Human. Er wurde validiert für WB, IHC, ELISA und FM.

Kurzübersicht für Vimentin Antikörper (ABIN6656056)

Target: Vimentin (VIM)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Klon: 2D1

Produktdetails anti-Vimentin Antikörper

Hersteller Produkt- Nr.: 200-301-e54

Hersteller: Rockland

Verwendungszweck: Vimentin Antibody

Aufreinigung: Anti-Vimentin Antibody was purified from tissue culture supernatant by protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-Vimentin Antibody was produced in mice by repeated immunizations with a recombinant human vimentin purified from E. coli.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:500-1:1000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Other: ICC: 1:500-1:2000

Kommentare:

Anti-Vimentin Antibody is tested for Western Blot, immunocytochemistry, and immunohistochemistry, and it is specific for the ~50 kDa vimentin protein. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Haltbarkeit: 12 months

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund:

Synonyms: Vim, Vimentin Antibody

Background: Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration. Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness. Vimentin Antibody is ideal for researchers interested in neuroscience research.

Gene Name: VIM

Gen-ID: 7431

NCBI Accession: NP_003371

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose