ENO2/NSE Antikörper

Produktnummer ABIN6656045

Hersteller Produkt- Nr.: 100-401-e00

Dieses Anti-ENO2/NSE-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ENO2/NSE in WB, IHC, IF, MA und FM. Geeignet für Human.

Kurzübersicht für ENO2/NSE Antikörper (ABIN6656045)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ENO2/NSE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Produktdetails anti-ENO2/NSE Antikörper

Hersteller Produkt- Nr.: 100-401-e00

Hersteller: Rockland

Verwendungszweck: Neuron specific enolase (NSE) Antibody

Kreuzreaktivität (Details): This antibody is a monospecific neat serum.

Aufreinigung: Anti-NSE antibody directed against human neuron specific enolase protein.

Immunogen: Anti-NSE Antibody was produced by repeated immunizations with recombinant human NSE expressed in E. coli.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:2000

Other: User Optimized

Kommentare:

Anti-Neuron specific enolase (Rabbit) antibody has been tested by Western Blot, IHC, ICC, and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47kDa in size corresponding to NSE protein by western blotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Andere Bezeichnung: Neuron specific enolase (NSE)

Hintergrund:

Synonyms: Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, NSE

Background: NSE antibody detects NSE protein. Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. Anti-neuron specific enolase (NSE) antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

Gene Name: ENO2

Gen-ID: 2026

UniProt: P09104