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TJP1 Antikörper

Dieses Anti-TJP1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TJP1 in WB, IHC, ELISA, IF, FACS, FM und MA. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.
Rockland
Produktnummer ABIN6655949
Hersteller Produkt- Nr.: 600-401-gu7

Kurzübersicht für TJP1 Antikörper (ABIN6655949)

Target

Alle TJP1 Antikörper anzeigen
TJP1 (Tight Junction Protein 1 (TJP1))

Reaktivität

  • 65
  • 39
  • 38
  • 20
  • 18
  • 9
  • 6
  • 6
  • 4
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 60
  • 6
  • 3
  • 1
  • 1
Kaninchen

Klonalität

  • 61
  • 10
Polyklonal

Konjugat

  • 47
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser TJP1 Antikörper ist unkonjugiert

Applikation

  • 59
  • 25
  • 22
  • 17
  • 17
  • 16
  • 14
  • 13
  • 13
  • 11
  • 8
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Multiplex Assay (MA)
  • Hersteller Produkt- Nr.

    600-401-gu7

    Hersteller

    Rockland

    Verwendungszweck

    ZO-1 Antibody

    Kreuzreaktivität (Details)

    This affinity purified antibody is directed against human ZO-1.

    Aufreinigung

    This product was affinity purified from monospecific antiserum by immunoaffinity purification.

    Sterilität

    Sterile filtered

    Immunogen

    Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

    Isotyp

    IgG
  • Applikationshinweise

    ELISA_Dilution: 10,000-1:50,000

    Immunohistochemistry_Dilution: 1:100-1:200

    Flow_Cytometry_Dilution: User Optimized

    IF_Microscopy_Dilution: 10 μg/mL

    Western_Blot_Dilution: 1:1000

    Kommentare

    Anti-ZO-1 Antibody has been tested in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. Expect a band at ~245 and/or 195.5 kDa in western blot using appropriate lysates. Positive control whole cell lysates used A549 and PC3 @ 1μg/mL for WB, CACO2 and PC3 with PFA and MeOH @ 10μg/mL for IF. Positive control cells for FC were PC3 and positive control tissues for IHC was mouse adipose tissue.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Singh, Zabbarova, Ikeda, Maranchie, Chermansky, Foley, Hitchens, Yoshimura, Kanai, Kaufman, Tyagi: "Virtual measurements of paracellular permeability and chronic inflammation via color coded pixel-wise T1 mapping." in: American journal of physiology. Renal physiology, Vol. 319, Issue 3, pp. F506-F514, (2020) (PubMed).

  • Target

    TJP1 (Tight Junction Protein 1 (TJP1))

    Andere Bezeichnung

    ZO-1

    Hintergrund

    Synonyms: rabbit anti-ZO-1 antibody, ZO 1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1

    Background: ZO-1, also called TJP1, belongs to the MAGUK family. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO-1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. ZO1 may be associated the following disorders, celiac desease, congenital nephrotic syndrome finnish type, and macular degeneration. Anti-ZO-1 Antibody is useful for researchers interested in Apoptosis Research and Insulin Research.

    Gene Name: TJP1

    Gen-ID

    7082

    NCBI Accession

    NP_003248

    UniProt

    Q07157

    Pathways

    Carbohydrate Homeostasis, Cell-Cell Junction Organization
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