KLF4 Antikörper (N-Term)

Produktnummer ABIN6655945

Produktdetails anti-KLF4 Antikörper

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KLF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Kreuzreaktivität: Human, Maus

Aufreinigung: This affinity purified antibody is directed against human KLF4. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and 93% identity to mouse.

Immunogen:

Immunogen: Anti-KLF4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Term portion of human KLF4 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200

Application Note: Anti-KLF4 Antibody has been tested in Western Blot, ELISA, and Immunofluorescence. Expect a band at ~54.7 kDa in western blot using appropriate lysates. Positive control whole cell lysates used K562 and PC3 @ 1 μg/mL for WB, and PC3 with PFA at 10 μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: User Optimized

Flow Cytometry Dilution: User Optimized

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 10 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Details zu KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Andere Bezeichnung: KLF4 (KLF4 Produkte)

Synonyme: EZF antikoerper, GKLF antikoerper, Gklf antikoerper, Zie antikoerper, KLF4 antikoerper, biklf antikoerper, biklf-A antikoerper, Kruppel like factor 4 antikoerper, Kruppel-like factor 4 (gut) antikoerper, Kruppel-like factor 4 antikoerper, Kruppel-like factor 4 (gut) L homeolog antikoerper, KLF4 antikoerper, Klf4 antikoerper, klf4 antikoerper, klf4.L antikoerper

Hintergrund:

Synonyms: rabbit anti-KLF4 Antibody, KLF-4 Antibody, Krueppel-like factor 4, Epithelial zinc finger protein EZF, EZF, Gut-enriched krueppel-like factor, GKLF, KLF 4

Background: KLF4 belong to the Kruppel like factors/Zinc fingers C2H2-type family. This gene encodes a protein that belongs to the Kruppel family of transcription factors. KLF4 can act both as activator and as repressor. It can bind the 5-CACCC-3 core sequence, the promoter region of its own gene, and can activate its own transcription. KLF4 regulates the expression of key transcription factors during embryonic development, playing an important role in maintaining embryonic stem cells, and in preventing their differentiation. This protein is required for normal development of the barrier function of skin postnatal, maintenance of the ocular surface, function in skeletal and kidney development, and thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. KLF4 may be associated with diseases such as Venous Hemangioma, Skin Squamous Cell Carcinoma, and pediatric lymphoma. Anti-KLF4 Antibody is useful for researchers interested in Peptide hormone metabolism, Stem Cell Differentiation Pathway Research, Wnt/Notch Pathway Research, and Cancer Research.

Gene Name: KLF4

Gen-ID: 9314

NCBI Accession: NP_001300981

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance