KLF4 Antikörper (N-Term)

Produktnummer ABIN6655944

Hersteller Produkt- Nr.: 600-401-gu2

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch KLF4 in WB, ELISA, IHC, IF, FACS, IP und FM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für KLF4 Antikörper (N-Term) (ABIN6655944)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KLF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Produktdetails anti-KLF4 Antikörper

Bindungsspezifität: N-Term

Hersteller Produkt- Nr.: 600-401-gu2

Hersteller: Rockland

Verwendungszweck: KLF4 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human KLF4.

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-KLF4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Term portion of human KLF4 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 5 μg/mL

Immunohistochemistry_Dilution: 1:200

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1 μg/mL

Kommentare:

Anti-KLF4 Antibody has been tested in Western Blot, ELISA, and Immunofluorescence. Expect a band at ~54.7 kDa in western blot using appropriate lysates. Positive control whole cell lysates NCCIT and PC3 @ 1μg/mL for WB, and PC3 with PFA at 10μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Andere Bezeichnung: KLF4

Hintergrund:

Synonyms: rabbit anti-KLF4 antibody, KLF-4 Antibody, Krueppel-like factor 4, Epithelial zinc finger protein EZF, EZF, Gut-enriched krueppel-like factor, GKLF, KLF 4

Background: KLF4 belong to the Kruppel like factors/Zinc fingers C2H2-type family. This gene encodes a protein that belongs to the Kruppel family of transcription factors. KLF4 can act both as activator and as repressor. It can bind the 5-CACCC-3 core sequence, the promoter region of its own gene, and can activate its own transcription. KLF4 regulates the expression of key transcription factors during embryonic development, playing an important role in maintaining embryonic stem cells, and in preventing their differentiation. This protein is required for normal development of the barrier function of skin postnatal, maintenance of the ocular surface, function in skeletal and kidney development, and thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. KLF4 may be associated with diseases such as Venous Hemangioma, Skin Squamous Cell Carcinoma, and pediatric lymphoma. Anti-KLF4 Antibody is useful for researchers interested in Peptide hormone metabolism, Stem Cell Differentiation Pathway Research, Wnt/Notch Pathway Research, and Cancer Research.

Gene Name: KLF4

Gen-ID: 9314

NCBI Accession: NP_001300981

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance