CES1 Antikörper

Produktnummer ABIN6655642

Hersteller Produkt- Nr.: 100-401-b14s

Der Kaninchen Polyklonal Anti-CES1-Antikörper wurde für WB, ELISA und IP validiert. Er ist geeignet, CES1 in Proben von Human zu detektieren.

Kurzübersicht für CES1 Antikörper (ABIN6655642)

Target: CES1 (Carboxylesterase 1 (CES1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CES1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-CES1 Antikörper

Hersteller Produkt- Nr.: 100-401-b14s

Hersteller: Rockland

Verwendungszweck: Liver Carboxylesterase-1 Antibody

Kreuzreaktivität (Details): The antibody detects recombinant hCE1 in cell lysates and native Liver Carboxylesterase-1 present in human tissue or fluids in various assays (ie.

Aufreinigung: This product was prepared from monospecific antiserum by delipidation and defibrination.

Sterilität: Sterile filtered

Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human Liver Carboxylesterase-1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: 1:100

ELISA_Dilution: 1:5,000 - 1:20,000

Western_Blot_Dilution: 1:2,500 - 1:5,000

Other: User Optimized

Kommentare:

This antibody has been tested for use in ELISA and western blotting and is specific for human CE1 (carboxylesterase).  Specific conditions for reactivity should be optimized by the end user.   Expect a band approximately 66 kDa in size corresponding CE1 by western blotting in the appropriate cell lysate or extract.  In western blot analysis endogenous protein present in human tissue or fluids, multiple bands of CE may appear corresponding to variable amount of glycosylation on the molecule.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 3 months

Details zu CES1

Target: CES1 (Carboxylesterase 1 (CES1))

Andere Bezeichnung: CES1

Hintergrund:

Synonyms: rabbit anti-Liver Carboxylesterase-1 Antibody, Liver carboxylesterase 1, Acyl coenzyme A:cholesterol acyltransferase, ACAT, Monocyte/macrophage serine esterase, HMSE, Serine esterase 1, Brain carboxylesterase hBr1, Triacylglycerol hydrolase, Egasyn, Retinyl ester hydrolase, REH

Background: Carboxylesterases (CES) are ubiquitous enzymes responsible for the hydrolysis of endogenous substrates with ester, thioester, or amide bonds and of xenobiotics (effecting detoxification or drug metabolism). Drug half-life and bioavailability may be modulated by selective inhibition of these proteins. CES1 or hCE1 (also known as acyl coenzyme A:cholesterol acyltransferase, monocyte/macrophage serine esterase, HMSE, serine esterase 1, brain carboxylesterase, triacylglycerol hydrolase, egasyn and retinyl ester hydrolase) is responsible for hydrolysis of stored cholesterol esters in macrophage foam cells and release of free cholesterol for high density lipoprotein-mediated cholesterol efflux.

Gene Name: CES1

Gen-ID: 1066, 68508965

UniProt: P23141

Pathways: Monocarboxylic Acid Catabolic Process