CD86 Antikörper (APC)

Produktnummer ABIN6655467

Hersteller Produkt- Nr.: 200-326-n75

Produktdetails anti-CD86 Antikörper (APC)

Target: CD86

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD86 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 200-326-n75

Hersteller: Rockland

Verwendungszweck: CD86 Allophycocyanin Antibody

Kreuzreaktivität (Details): Reactivity is observed against human CD86.

Aufreinigung: Allophycocyanin conjugated CD86 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD86.

Sterilität: Sterile filtered

Immunogen: Anti-CD86 Antibody (Monoclonal) was produced by repeated immunizations with CD86 antigen.

Klon: IT2-2

Isotyp: IgG2b kappa

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 uL/test/1x10e5 to 1x10e8 cells

Western_Blot_Dilution: User Optimized

Kommentare:

Anti-CD86 is tested for Flow Cytometry and useful in Immunohistochemistry and Western Blot. Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu CD86

Target: CD86

Andere Bezeichnung: CD86 (CD86 Produkte)

Hintergrund:

Synonyms: T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD28LG2

Background: CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Gene Name: CD86

Gen-ID: 942

NCBI Accession: NP_001193853

UniProt: P42081

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation