Beclin 1 Antikörper (pSer234)

Produktnummer ABIN6655419

Hersteller Produkt- Nr.: 600-401-w96

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Beclin 1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für Beclin 1 Antikörper (pSer234) (ABIN6655419)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Beclin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Beclin 1 Antikörper

Bindungsspezifität: pSer234

Hersteller Produkt- Nr.: 600-401-w96

Hersteller: Rockland

Verwendungszweck: BECLIN1 phospho S234 Antibody

Kreuzreaktivität (Details): Anti-Beclin1 pS234 Antibody is directed against Beclin 1 protein phosphorylated at S234.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Anti-Phospho-Ser234 Beclin-1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of human Beclin-1 conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:2,500

Western_Blot_Dilution: 1:250

Kommentare:

Suggested Applications: ELISA
Anti-Phospho-Ser234 Beclin-1 Antibody is tested for WB. Expect a band at approximately ~60 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user. Immunolabeling of the Beclin-1 band is decreased in the Beclin-1 alanine substitution mutant.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-Beclin 1 Antikörper (ABIN6655419)

Xie, Kang, Tang: "Assessment of Posttranslational Modifications of ATG proteins." in: Methods in enzymology, Vol. 587, pp. 171-188, (2017) (PubMed).

Details zu Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Andere Bezeichnung: Beclin1

Hintergrund:

Synonyms: Run domain Beclin-1 interacting and cysteine-rich containing protein, Rubicon, Beclin-1 associated RUN domain containing protein

Background: Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies. Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser234. Beclin 1 pS234 Antibody is ideal for researchers interested in cancer research.

Gene Name: BECN1

Gen-ID: 8678

NCBI Accession: NP_001300927

UniProt: Q14457

Pathways: Autophagie