LSD1 Antikörper

Produktnummer ABIN6655416

Hersteller Produkt- Nr.: 200-401-w52

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LSD1 in WB und ChIP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für LSD1 Antikörper (ABIN6655416)

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LSD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-LSD1 Antikörper

Hersteller Produkt- Nr.: 200-401-w52

Hersteller: Rockland

Verwendungszweck: LSD1 Antibody

Kreuzreaktivität (Details): Cross reactivity with other species was not tested.

Aufreinigung: Anti-LSD1 Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-LSD1 Antibody was produced in rabbits by repeated immunizations with a full length recombinant protein of human LSD1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ChIP_Dilution: 3 μg per IP

Western_Blot_Dilution: 1:1,000

Kommentare:

Anti-LSD1 Antibody is tested for Chromatin Immunoprecipitation and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 110 kDa in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Konservierungsmittel: ProClin, Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu LSD1

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Andere Bezeichnung: LSD1

Hintergrund:

Synonyms: Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, AOF2, KDM1

Background: LSD1 (lysine specific demethylase 1) is a component of the histone demethylase complex that uses FAD as a prosthetic goup. LSD1 may have a dual effect on gene transcription. As it demethylates the mono- and dimethylated 'Lys-4' of histone H3, which are associated with transcriptional activation, LSD1 can act as a repressor of gene expression. However, LSD1 is also capable of demethylating 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to activation of androgen receptor target genes. LSD1 therefore is ideal for research in different processes such as Stem and Cancer Cell Biology, Embryonic Development, Epigenetics and Cell Differentiation and Proliferation.

Gene Name: KDM1A

Gen-ID: 23028

NCBI Accession: NP_001009999

UniProt: O60341

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effekt