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POLR2A/RPB1 Antikörper (pSer5)

Dieses Anti-POLR2A/RPB1-Antikörper ist ein Mouse Monoclonal-Antikörper zur Detektion von POLR2A/RPB1 in WB, IF, ELISA, ChIP, FM. Geeignet für Human.
Rockland
Produktnummer ABIN6655367
Hersteller Produkt- Nr.: 200-301-v01

Kurzübersicht für POLR2A/RPB1 Antikörper (pSer5) (ABIN6655367)

Target

Alle POLR2A/RPB1 (POLR2A) Antikörper anzeigen
POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reaktivität

  • 97
  • 42
  • 41
  • 6
  • 6
  • 6
  • 6
  • 5
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Human

Wirt

  • 77
  • 21
  • 1
  • 1
  • 1
Maus

Klonalität

  • 52
  • 49
Monoklonal

Konjugat

  • 70
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 1
  • 1
Dieser POLR2A/RPB1 Antikörper ist unkonjugiert

Applikation

  • 64
  • 35
  • 34
  • 31
  • 22
  • 10
  • 10
  • 9
  • 6
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
  • Bindungsspezifität

    • 20
    • 18
    • 11
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer5

    Hersteller Produkt- Nr.

    200-301-v01

    Hersteller

    Rockland

    Verwendungszweck

    POL II phospho S5 Antibody

    Kreuzreaktivität (Details)

    This antibody is specific for Pol II protein phosphorylated Ser5.

    Aufreinigung

    Anti-Pol II pS5 Antibody was purified by Protein A chromatography.

    Sterilität

    Sterile filtered

    Immunogen

    Anti-Pol II S5p Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II phosphorylated at Ser5 of the repeated sequence.

    Isotyp

    IgG1
  • Applikationshinweise

    ChIP_Dilution: 1 μg/ChIP

    ELISA_Dilution: 1:3,000

    IF_Microscopy_Dilution: 1:500

    Western_Blot_Dilution: 1:1,000

    Kommentare

    Anti-Pol II pS5 Antibody is tested for Chromatin Immunoprecipitation Sequencing, Chromatin Immunoprecipitation, ELISA, Immunofluorescence and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 225 kDa in the appropriate cell lysate or extract.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.05 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

    Andere Bezeichnung

    Pol II

    Hintergrund

    Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

    Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II S5p Antibody is ideal for research in Gene Expression, Transcription and Genetics.

    Gene Name: POLR2A

    Gen-ID

    5430

    NCBI Accession

    NP_000928

    UniProt

    P24928

    Pathways

    Regulatorische RNA Pathways
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