POLR2A/RPB1 Antikörper (pSer2)

Produktnummer ABIN6655366

Hersteller Produkt- Nr.: 200-301-v00

Der Maus Monoklonal Anti-POLR2A/RPB1-Antikörper wurde für WB, IF, ELISA, ChIP und FM validiert. Er ist geeignet, POLR2A/RPB1 in Proben von Human zu detektieren.

Kurzübersicht für POLR2A/RPB1 Antikörper (pSer2) (ABIN6655366)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser POLR2A/RPB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Produktdetails anti-POLR2A/RPB1 Antikörper

Bindungsspezifität: pSer2

Hersteller Produkt- Nr.: 200-301-v00

Hersteller: Rockland

Verwendungszweck: POL II phospho S2 Antibody

Kreuzreaktivität (Details): This antibody is specific for Pol II protein phosphorylated Ser2.

Aufreinigung: Anti-Pol II pS2 Antibody was purified by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-Pol II S2p Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II phosphorylated at Ser2 of the repeated sequence.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ChIP_Dilution: 1 μg/ChIP

ELISA_Dilution: 1:3,000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:1,000

Kommentare:

Anti-Pol II pS2 Antibody is tested for Chromatin Immunoprecipitation Sequencing, Chromatin Immunoprecipitation, ELISA, Immunofluorescence and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 225 kDa in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Andere Bezeichnung: Pol II

Hintergrund:

Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit (UniProt/Swiss-Prot entry P24928) is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II S2p Antibody is ideal for research in Gene Expression, Transcription, and Genetics.

Gene Name: POLR2A

Gen-ID: 5430

NCBI Accession: NP_000928

UniProt: P24928

Pathways: Regulatorische RNA Pathways