Progesterone Receptor Antikörper (pSer294)

Produktnummer ABIN6655216

Hersteller Produkt- Nr.: 209-301-e17

Der Maus Monoklonal Anti-Progesterone Receptor-Antikörper wurde für WB und IHC validiert. Er ist geeignet, Progesterone Receptor in Proben von Human zu detektieren.

Kurzübersicht für Progesterone Receptor Antikörper (pSer294) (ABIN6655216)

Target: Progesterone Receptor (PGR)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Progesterone Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 608

Produktdetails anti-Progesterone Receptor Antikörper

Bindungsspezifität: pSer294

Hersteller Produkt- Nr.: 209-301-e17

Hersteller: Rockland

Verwendungszweck: Progesterone Receptor phospho S294 Antibody

Aufreinigung: Anti-Progesterone Receptor pS294 (Mouse) Monoclonal Antibody is Protein G purified from cell culture supernatant. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Immunogen: Anti-Progesterone Receptor pS294 (Mouse) Monoclonal Antibody was produced in mouse by repeated immunizations with Progesterone Receptor pS294- Synthetic peptide corresponding to amino acid residues surrounding Ser294 of the human progesterone receptor protein.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Progesterone Receptor pS294 (Mouse) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should but optimized by the end user. Expect a band of approximately 90 kDa in size for PR-A isoform and the approximately 120 kDa PR-B isoform phosphorylated at Ser294.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Progesterone Receptor

Target: Progesterone Receptor (PGR)

Andere Bezeichnung: Progesterone Receptor

Hintergrund:

Synonyms: mouse anti-Progesterone Receptor phospho S294 Antibody, mouse anti-Progesterone Receptor pS294 antibody, PR, Nuclear receptor subfamily 3 group C member 3

Background: There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones. Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor. Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor. Anti-Progesterone Receptor pS294 (Mouse) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Cancer research.

Gene Name: PGR

Gen-ID: 5241

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration