Progesterone Receptor Antikörper (AA 412-526)

Produktnummer ABIN6655214

Produktdetails anti-Progesterone Receptor Antikörper

Target: Progesterone Receptor (PGR)

Bindungsspezifität: AA 412-526

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Progesterone Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Aufreinigung: This product is clarified tissue culture supernate. The antibody is specific for human Progesterone Receptor protein. Cross-reactivity with Progesterone Receptor protein from other sources has not been determined.

Immunogen:

Immunogen: Anti-Progesterone Receptor monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 412-526 of human Progesterone Receptor protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.

Immunogen Type: Recombinant Protein

Klon: SP2

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:400

Application Note: This monoclonal antibody contains TBS/1 % BSA and is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 116 kDa in size corresponding to PgR isoform B (the 'canonical' sequence) protein by western blotting in the appropriate cell lysate or extract. Progesterone Receptor is found in human Breast ductal carcinoma and is a nuclear protein. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:200-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date.

Referenzen für anti-Progesterone Receptor Antikörper (ABIN6655214)

Huang, Zhu, Szekeres, Xia: "Development of new rabbit monoclonal antibody to estrogen receptor: immunohistochemical assessment on formalin-fixed, paraffin-embedded tissue sections." in: Applied immunohistochemistry & molecular morphology : AIMM, Vol. 13, Issue 1, pp. 91-5, (2005) (PubMed).

Cano, Milanezi, Leitão, Ricardo, Brito, Schmitt: "Estimation of hormone receptor status in fine-needle aspirates and paraffin-embedded sections from breast cancer using the novel rabbit monoclonal antibodies SP1 and SP2." in: Diagnostic cytopathology, Vol. 29, Issue 4, pp. 207-11, (2004) (PubMed).

Details zu Progesterone Receptor

Target: Progesterone Receptor (PGR)

Andere Bezeichnung: Progesterone Receptor (PGR Produkte)

Synonyme: NR3C3 antikoerper, PR antikoerper, 9930019P03Rik antikoerper, BB114106 antikoerper, ENSMUSG00000074510 antikoerper, PR-A antikoerper, PR-B antikoerper, XPR-1 antikoerper, pgr antikoerper, xpr-2 antikoerper, LOC443444 antikoerper, PGR antikoerper, pg antikoerper, pr antikoerper, gb:dq017620 antikoerper, progesterone receptor antikoerper, progesterone receptor L homeolog antikoerper, PGR antikoerper, Pgr antikoerper, pgr.L antikoerper, pgr antikoerper

Hintergrund:

Synonyms: rabbit anti-Progesterone Receptor Antibody, PgR, PR, nuclear receptor subfamily 3 group C member 3

Background: The PGR gene encodes a member of the steroid receptor superfamily referred to as progesterone receptor (also known as PgR, PR and nuclear receptor subfamily 3 group C member 3). Progesterone receptor mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform A only, and mediate their own response genes and physiologic effects with little overlap. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.

Gene Name: PGR

Gen-ID: 5241

UniProt: P06401

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration