CD3 epsilon Antikörper (PE)

Produktnummer ABIN6655180

Hersteller Produkt- Nr.: 200-308-n12

Der Maus Monoklonal anti-CD3 epsilon Antikörper wird verwendet zum Nachweis von CD3 epsilon in Proben von Human. Er wurde validiert für FACS.

Kurzübersicht für CD3 epsilon Antikörper (PE) (ABIN6655180)

Target: CD3 epsilon (CD3E)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: HIT3a

Produktdetails anti-CD3 epsilon Antikörper (PE)

Hersteller Produkt- Nr.: 200-308-n12

Hersteller: Rockland

Verwendungszweck: CD3 Phycoerythrin Antibody

Kreuzreaktivität (Details): Reactivity is observed against human CD3.

Aufreinigung: Phycoerythrin conjugated CD3 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD3.

Sterilität: Sterile filtered

Immunogen: Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.

Isotyp: IgG2a kappa

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise: Optional[Neutralization_Dilution]: 5 uL/test/1x10e5 to 1x10e8 cells

Kommentare:

Anti-CD3 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3E

Hintergrund:

Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

Background: The Hit3a antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor - CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The Hit3a antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of Hit3a antibody to CD3e can induce cell activation. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3.

Gene Name: CD3E

Gen-ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway