CD3 epsilon Antikörper (FITC)

Produktnummer ABIN6655177

Hersteller Produkt- Nr.: 200-302-n12

Der Maus Monoklonal anti-CD3 epsilon Antikörper wird verwendet zum Nachweis von CD3 epsilon in Proben von Human. Er wurde validiert für FACS, IHC und IP.

Kurzübersicht für CD3 epsilon Antikörper (FITC) (ABIN6655177)

Target: CD3 epsilon (CD3E)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Klon: HIT3a

Produktdetails anti-CD3 epsilon Antikörper (FITC)

Hersteller Produkt- Nr.: 200-302-n12

Hersteller: Rockland

Verwendungszweck: CD3 Fluorescein Antibody

Kreuzreaktivität (Details): Reactivity is observed against human CD3.

Aufreinigung: Fluorescein conjugated CD3 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD3.

Sterilität: Sterile filtered

Immunogen: Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.

Isotyp: IgG2a kappa

Labeling Ratio: 4-6

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Kommentare:

Anti-CD3 has been tested in Flow Cytometry and is useful for Immunoprecipitation, and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Haltbarkeit: 6 months

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3E

Hintergrund:

Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

Background: CD3 epsilon is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3e, one CD3 gamma, one CD3 delta, one CD3 zeta (CD247), and a T-cell receptor ( alpha/beta or gamma/delta) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Gene Name: CD3E

Gen-ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway