CD3 epsilon Antikörper (APC)

Produktnummer ABIN6655175

Hersteller Produkt- Nr.: 200-326-n12

Der APC-konjugierte Maus Monoklonal anti-CD3 epsilon Antikörper (Klon HIT3a) (ABIN6655175) detektiert spezifisch CD3 epsilon in FACS, IHC und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CD3 epsilon Antikörper (APC) (ABIN6655175)

Target: CD3 epsilon (CD3E)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Klon: HIT3a

Produktdetails anti-CD3 epsilon Antikörper (APC)

Hersteller Produkt- Nr.: 200-326-n12

Hersteller: Rockland

Verwendungszweck: CD3 Allophycocyanin Antibody

Kreuzreaktivität (Details): Reactivity is observed against human CD3.

Aufreinigung: Allophycocyanin conjugated CD3 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD3.

Sterilität: Sterile filtered

Immunogen: Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.

Isotyp: IgG2a kappa

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Kommentare:

Anti-CD3 is tested in Flow Cytometry and useful for Immunoprecipitation, Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3E

Hintergrund:

Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

Background: CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Gene Name: CD3E

Gen-ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway