CD47 Antikörper (FITC)

Produktnummer ABIN6655123

Hersteller Produkt- Nr.: 200-302-m68

Der Maus Monoklonal Anti-CD47-Antikörper wurde für FACS und IHC validiert. Er ist geeignet, CD47 in Proben von Human zu detektieren.

Kurzübersicht für CD47 Antikörper (FITC) (ABIN6655123)

Target: CD47

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD47 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Klon: B6H12-2

Produktdetails anti-CD47 Antikörper (FITC)

Hersteller Produkt- Nr.: 200-302-m68

Hersteller: Rockland

Verwendungszweck: CD47 Fluorescein Antibody

Kreuzreaktivität (Details): Reactivity is observed against human CD47.

Aufreinigung: Fluorescein conjugated CD47 Monoclonal Antibody was Protein G Purified and is directed against human CD47.

Sterilität: Sterile filtered

Immunogen: Anti-CD47 Antibody (Monoclonal) was produced by repeated immunizations with CD47 antigen.

Isotyp: IgG1

Labeling Ratio: 2-8

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/5 x 10^5 cells (0.5 μg)

Kommentare:

Anti-CD47 has been tested in Flow Cytometry (Cell Surface) and is useful for Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Optional[Buffer]: 0.01 M Tris Cl, 0.15 M Sodium Chloride, pH 8.0 ,0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Haltbarkeit: 6 months

Details zu CD47

Target: CD47

Andere Bezeichnung: CD47

Hintergrund:

Synonyms: Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47

Background: CD47, originally named integrin-associated protein (IAP), is a 50- kDa protein containing five membrane spanning sequences and a short cytoplasmic tail. CD47 appears to be important in myeloid cell activation and migration across endothelial and epithelial monolayers. Anti-CD47 antibody has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47 and its ligands, thrombospondin 1 (TSP-1) and SIRP-a, are important regulators of dendritic cells. Recently, it has been shown that CD47 and TLR2 cross-talk to regulate PMN transmigration. The activation of TLR2/6 by MALP-2, a TLR2 specific agonist, potently inhibits human and murine PMN transmigration. The cross-talk between TLR2 and CD47 may be mediated through MYD88.

Gene Name: CD47

Gen-ID: 961

NCBI Accession: NP_001768

UniProt: Q08722