Androgen Receptor Antikörper (N-Term, Ser213)

Produktnummer ABIN6655076

Hersteller Produkt- Nr.: 200-301-h54

Dieses Anti-Androgen Receptor-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Androgen Receptor in WB und IHC. Geeignet für Human.

Kurzübersicht für Androgen Receptor Antikörper (N-Term, Ser213) (ABIN6655076)

Target: Androgen Receptor (AR)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Androgen Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 156C135-2

Produktdetails anti-Androgen Receptor Antikörper

Bindungsspezifität: N-Term, Ser213

Hersteller Produkt- Nr.: 200-301-h54

Hersteller: Rockland

Verwendungszweck: Androgen Receptor Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Androgen Receptor from human and New World Monkey on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Androgen Receptor Antibody was purified by Protein G chromatography.

Immunogen: Androgen Receptor Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids near the n-terminus of the Androgen Receptor containing the serine 213 phosphorylated serine in the peptide sequence.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1-4 μg/mL

Kommentare:

Anti-Androgen Receptor Antibody is tested for use in IHC (p) and WB. Expect a band approximately 110kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Androgen Receptor

Target: Androgen Receptor (AR)

Andere Bezeichnung: Androgen Receptor

Hintergrund:

Synonyms: DHTR, NR3C4

Background: Anti-Androgen Receptor Antibody detects human Androgen receptor. The androgen receptor (AR) is an ~110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. IGF-1 activates the phosphatidylinositol 3-kinase(PI3K)/AKT pathway in LNCap at high passage number and increases phosphorylation of of AR at Ser213/210 (see western blot) and Ser791/790. The western blot results also show that inhibition of the PI3K/Akt pathway by LY294002 prior to incubation with IGF-1 suppressed AR phosphorylation at Ser213/210. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis. Anti-Androgen Receptor Antibody is ideal for investigators involved in neuroscience, cytokines and growth factors, and transcription factors.

Gene Name: AR

Gen-ID: 367

NCBI Accession: NP_000035

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding