EPH Receptor B2 Antikörper (pTyr331)

Produktnummer ABIN6655037

Hersteller Produkt- Nr.: 600-401-d37

Produktdetails anti-EPH Receptor B2 Antikörper

Target: EPH Receptor B2 (EPHB2)

Bindungsspezifität: pTyr331

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EPH Receptor B2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 600-401-d37

Hersteller: Rockland

Verwendungszweck: EphrinB phospho Y331 Antibody

Kreuzreaktivität (Details): Anti-EphrinB pY331 antibody is directed against rat EphrinB.

Aufreinigung: Anti-EphrinB pY331 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-EphrinB pY331 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr331 Xenopus EphrinB conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-EphrinB pY331 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 46 kDa in size corresponding to EphrinB protein phosphorylated at Tyr331 in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Andere Bezeichnung: EPHB2 (EPHB2 Produkte)

Hintergrund:

Synonyms: Ephrin type-B receptor 2, EPH-like kinase 5, EK5

Background: EphrinB Antibody detects EphrinB proteins which are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of EphrinB was phosphorylated in HEK293 cells after timulation by the soluble EphB2 receptor tyrosine kinase. Anti-EphrinB pY331 Antibody is ideal for investigators involved in Cell Migration, Cell Division, Cell Signaling and Neuroscience.

Gene Name: EPHB2

Gen-ID: 396513

NCBI Accession: NP_996834

UniProt: P28693

Pathways: RTK Signalweg, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteine