Acetylated Lysine Antikörper

Produktnummer ABIN6655030

Hersteller Produkt- Nr.: 200-301-a97

Der Maus Monoklonal anti-Acetylated Lysine Antikörper wird verwendet zum Nachweis von Acetylated Lysine. Er wurde validiert für WB, ELISA und IHC.

Kurzübersicht für Acetylated Lysine Antikörper (ABIN6655030)

Target: Acetylated Lysine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Acetylated Lysine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Klon: 7F8

Produktdetails anti-Acetylated Lysine Antikörper

Hersteller Produkt- Nr.: 200-301-a97

Hersteller: Rockland

Verwendungszweck: Lysine Acetylated Antibody

Kreuzreaktivität (Details): This antibody is known to react with acetylated lysine residues from mammalian and avian sources.

Aufreinigung: This Protein G purified monoclonal antibody reacts with proteins containing acetylated lysine residues and does not detect non-acetylated lysine residues.

Sterilität: Sterile filtered

Immunogen: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with acetylated keyhole limpet hemocyanin (KLH).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:200 - 1:1,000

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Kommentare:

This Protein G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Approximately 1 μg of antibody is sufficient to detect 20 μg of acetylated chicken erythrocyte histones (sodium butyrate treated) by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110). Antibody provided in PBS pH 7.4.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: See application note.

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Acetylated Lysine

Target: Acetylated Lysine

Andere Bezeichnung: Lysine Acetylated

Substanzklasse: Chemical

Hintergrund:

Synonyms: acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody

Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. A common posttranscriptional modification of lysine residues involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4). HDACs therefore are promising targets for anticancer drugs currently in development (5).