Acetylated Lysine Antikörper

Produktnummer ABIN479096

Der Maus Monoklonal anti-Acetylated Lysine Antikörper wird verwendet zum Nachweis von Acetylated Lysine. Er wurde validiert für WB und IP.

Kurzübersicht für Acetylated Lysine Antikörper (ABIN479096)

Target: Acetylated Lysine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Acetylated Lysine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-Acetylated Lysine Antikörper

Spezifität: Recognizes acetylated lysine-containing proteins including histones. Of all the possible H3 and H4 acetylation sites, this antibody exhibits highest affinity for histone H4 acetylated on lysine 8 and histone H3 acetylated on lysine 14.

Aufreinigung: Protein G purified

Immunogen: Mixture of chemically acetylated antigens.

Type of Immunogen: Hapten

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP (0.5 - 2 μg/mL), WB

Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 μg immunoprecipitates in vitro acetylated PCAF added to a 3T3 RIPA cell lysate. The immunoprecipitated PCAF was detected by subsequent immunoblot analysis using 1 μg/mL monoclonal anti-GST.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 70 % storage buffer (0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide), 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Acetylated Lysine

Target: Acetylated Lysine

Andere Bezeichnung: Acetyl-Lysine

Substanzklasse: Chemical