CRYAB Antikörper

Produktnummer ABIN6654631

Der Maus Monoklonal Anti-CRYAB-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, CRYAB in Proben von Human und Ratte zu detektieren.

Kurzübersicht für CRYAB Antikörper (ABIN6654631)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CRYAB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CPTC-CRYAB-1

Produktdetails anti-CRYAB Antikörper

Aufreinigung: Purified

Reinheit: Protein G affinity chromatography

Immunogen: A full length recombinant human protein was used as the immunogen for the CRYAB antibody.

Isotyp: IgG2c

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CRYAB antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CRYAB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Andere Bezeichnung: CRYAB

Hintergrund: Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into alpha, beta and gamma families, and the beta- and gamma-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha-crystallins consist of three gene products, alpha-A-, alpha-B- and alpha-C-crystallin, which are members of the small heat shock protein family (HSP 20). Alpha-crystallins act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, alpha-crystallins do not renature these proteins. Expression of alpha-A-crystallin is restricted to the lens and defects of this gene cause the development of autosomal dominant congenital cataracts (ADCC). The human alpha-B-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of alpha-B-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.