Rekombinanter BrdU Antikörper

Produktnummer ABIN6654472

Der Kaninchen Monoklonal anti-BrdU Antikörper wird verwendet zum Nachweis von BrdU. Er wurde validiert für IHC (p).

Kurzübersicht für Rekombinanter BrdU Antikörper (ABIN6654472)

Target: BrdU (Bromodeoxyuridine (BrdU))

Antikörpertyp: Recombinant Antibody

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser BrdU Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: BRD2888R

Produktdetails anti-BrdU Antikörper

Verwendungszweck: BrdU Antibody / Bromodeoxyuridine (azide and preservative free)

Aufreinigung: Protein A affinity chromatography

Immunogen: BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this BrdU antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.

1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Andere Bezeichnung: BrdU

Substanzklasse: Chemical

Hintergrund: This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.