BrdU Antikörper

Produktnummer ABIN6654472

Der Kaninchen Monoklonal Anti-BrdU-Antikörper wurde für IHC (p) validiert. Er ist geeignet, BrdU in Proben von zu detektieren.

Kurzübersicht für BrdU Antikörper (ABIN6654472)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser BrdU Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: BRD2888R

Produktdetails anti-BrdU Antikörper

Aufreinigung: Purified

Reinheit: Protein A affinity chromatography

Immunogen: BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Andere Bezeichnung: BrdU

Substanzklasse: Chemical

Hintergrund: This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.